The Cathedral was once the seat of the bishops of Dunblane (also sometimes called 'of Strathearn'), until the abolition of bishops after the Scottish Reformation.

There are remains of the vaults of the episcopal palace to the south of the cathedral.

Technically, it is no longer a cathedral, as there are no bishops in the Church of Scotland, which is a Presbyterian denomination.

William Chisholme, the last Catholic bishop of Dunblane in 1561, later became bishop of Vaison in France.

It contains the graves of Margaret Drummond of Stobhall, a mistress of King James IV of Scotland and her two sisters, all said to have been poisoned.

The building is largely 13th century in date, though it incorporates an originally free-standing bell-tower of 11th century date on its south side. This tower was increased in height in the 15th century, a change clearly visible in the colour of the stonework, and in the late gothic style of the upper storey's windows.