The structure of the present building dates from this time. In 1377, the manor of Langley passed to the Lee family, who fitted out the chapel in about 1546. In 1591, Sir Humphrey Lee moved to Langley. He was probably responsible for re-roofing the chapel in 1601 – a date given on the nave roof – and refurnishing it a few years later.

By 1700 the chapel had largely fallen out of use, and thus escaped any attempt to re-order the interior according to the more elaborate ‘High Church’ model favoured in the 19th century. The last regular service was held here in 1871.